BioChem - DOS

Biohydrogen and 5-aminolevulinic acid production from waste barley by Rhodobacter sphaeroides O.U.001 in a biorefinery concept

International journal of hydrogen energy

Kars, G.; Ceylan, A.

The production of biohydrogen and 5-aminolevulinic acid (5-ALA) by Rhodobacter sphaeroides O.U.001 was investigated in a biorefinery concept. Waste barley was used as a substrate after acid hydrolysis. The hydrolysate was analyzed in terms of its total simple sugar, organic acid, ammonium, element and total phenol contents. Four different growth media having 5 g/L, 7 g/L, 9 g/L and 11 g/L sugar content were prepared using the waste barley hydrolysate to produce biohydrogen and 5-ALA. The increased sugar concentrations resulted in higher cell density and hydrogen accumulation. Accordingly, the highest cell density (OD<SUB>660</SUB>: 1.78) and hydrogen production (0.4 L H<SUB>2</SUB>/L culture) were observed in the 11 g/L sugar-containing medium. A 67.4 &mu;M 5-ALA was produced upon vitamin B<SUB>12</SUB> and levulinic acid additions. These results showed that waste barley can be used as a substrate for R. sphaeroides for biohydrogen and 5-ALA production within a biorefinery concept.

2013

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

38

14

pp.5573-5579

10.1016/j.ijhydene.2013.03.013

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65883611

Biohydrogen; Aminolevulinic acid; Rhodobacter sphaeroides; Waste barley; Biorefinery concept