초록
<P><B>Abstract</B></P> <P>The pathway engineering of <I>Enterobacter aerogenes</I> was attempted to improve its production capability of 2,3–butanediol from lignocellulosic biomass. In the medium containing glucose and xylose mixture as carbon sources, the gene deletion of <I>pflB</I> improved 2,3-butanediol carbon yield by 40%, while the deletion of <I>ptsG</I> increased xylose consumption rate significantly, improving the productivity at 12 hr by 70%. The constructed strain, EMY-22-galP, overexpressing glucose transporter (<I>galP</I>) in the triple gene knockout <I>E. aerogenes</I>, <I>ldhA</I>, <I>pflB</I>, and <I>ptsG</I>, provided the highest 2,3-butanediol titer and yield at 12 hr flask cultivation. Sugarcane bagasse was pretreated with green liquor, a solution containing Na<SUB>2</SUB>CO<SUB>3</SUB> and Na<SUB>2</SUB>SO<SUB>3</SUB> and was hydrolyzed by enzymes. The resulting hydrolysate was used as a carbon source for 2,3-butanediol production. After 72 hr in fermentation, the yield of 0.395g/g sugar was achieved, suggesting an economic production of 2,3-butanediol was possible from lignocellulosic biomass with the metabolically engineered strain.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Enterobacter aerogenes</I> was engineered for utilization of lignocellulosic biomass. </LI> <LI> The deletion of <I>pflB</I> improved the carbon yield of 2,3-butanediol from the sugar. </LI> <LI> The deletion of <I>ptsG</I> increased xylose consumption rate for 2,3-butanediol production. </LI> <LI> Developed strain performed well with sugarcane bagasse hydrolysates in fermentation. </LI> </UL> </P>