초록
<P><B>Abstract</B></P> <P> <I>Clostridium ljungdahlii</I> has emerged as an attractive candidate for the bioconversion of synthesis gas (CO, CO<SUB>2</SUB>, H<SUB>2</SUB>) to a variety of fuels and chemicals through the Wood-Ljungdahl pathway. However, metabolic engineering and pathway elucidation in this microbe is limited by the lack of genetic tools to downregulate target genes. To overcome this obstacle, here we developed an inducible CRISPR interference (CRISPRi) system for <I>C. ljungdahlii</I> that enables efficient (> 94%) transcriptional repression of several target genes, both individually and in tandem. We then applied CRISPRi in a strain engineered for 3-hydroxybutyrate (3HB) production to examine targets for increasing carbon flux toward the desired product. Downregulating phosphotransacetylase (<I>pta</I>) with a single sgRNA led to a 97% decrease in enzyme activity and a 2.3-fold increase in titer during heterotrophic growth. However, acetate production still accounted for 40% of the carbon flux. Repression of aldehyde:ferredoxin oxidoreductase (<I>aor2</I>), another potential route for acetate production, led to a 5% reduction in acetate flux, whereas using an additional sgRNA targeted to <I>pta</I> reduced the enzyme activity to 0.7% of the wild-type level, and further reduced acetate production to 25% of the carbon flux with an accompanying increase in 3HB titer and yield. These results demonstrate the utility of CRISPRi for elucidating and controlling carbon flow in <I>C. ljungdahlii</I>.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A CRISPRi system is developed for <I>Clostridium ljungdahlii.</I> </LI> <LI> Use of a tetracycline promoter allows inducible downregulation with CRISPRi. </LI> <LI> Downregulation of <I>pta</I> increases 3HB production 2.6-fold under heterotrophic conditions. </LI> <LI> Heterotrophic acetate production is partially mediated by aldehyde:ferredoxin oxidoreductase (<I>aor</I>) in <I>Clostridium ljungdahlii</I>. </LI> </UL> </P>