초록
<P>The immobilized cellulase-producing mycelium of <I>Trichoderma reesei</I> was found to produce 2.9 U/ml of cellulase activity within 144 h while 2.1 U/ml of cellulase activity was produced within 120 h by the free mycelium of the same strain. When the immobilized mycelium of <I>T</I>. <I>reesei</I> was co-cultivated with the free cells of <I>Yarrowia lipolytica</I> SWJ-1b in flask, <I>Y</I>. <I>lipolytica</I> SWJ-1b could yield 10.7 g/l of citric acid and 3.9 g/l of isocitric acid from 40.0 g/l pretreated straw within 240 h. Under the similar conditions, <I>Y</I>. <I>lipolytica</I> SWJ-1b could yield 32.8 g/l of citric acid and 4.7 g/l of isocitric acid from 40.0 g/l pretreated straw supplemented with 20.0 g/l glucose within 288 h. When the co-cultures were grown in 10-l fermentor, <I>Y</I>. <I>lipolytica</I> SWJ-1b could yield 83.4 g/l of citric acid and 8.7 g/l of isocitric acid from 100.0 g/l of pretreated straw supplemented with 50.0 g/l glucose within 312 h.</P>