Development of an engineered thermostable amine dehydrogenase for the synthesis of structurally diverse chiral amines

BioChem - DOS

Search

메타 데이터

바이오화학분류

- 바이오정밀화학
  1. 기타
- 화장품용 기능성소재
  1. 기능성

논문

Development of an engineered thermostable amine dehydrogenase for the synthesis of structurally diverse chiral amines

학술지

Catalysis science & technology

저자명

Liu, Lei; Wang, Dong-Hao; Chen, Fei-Fei; Zhang, Zhi-Jun; Chen, Qi; Xu, Jian-He; Wang, Zhi-Long; Zheng, Gao-Wei

초록

Amine dehydrogenases (AmDHs) are emerging as a class of attractive biocatalysts for synthesizing chiral aminesviaasymmetric reductive amination of ketones with inexpensive ammonia as an amino donor. However, the AmDHs developed to date exhibit limited substrate scope. Here, using directed evolution, we engineered aGkAmDH based on a thermostable phenylalanine dehydrogenase fromGeobacillus kaustophilus. The newly developed AmDH is able to catalyze reductive amination of a diverse set of ketones and functionalized hydroxy ketones with ammonia or primary amines with up to &gt;99% conversion, thus accessing structurally diverse chiral primary and secondary amines and chiral vicinal amino alcohols, with excellent enantioselectivity (up to &gt;99% ee) and releasing water as the sole by-product.

발행연도

2020

발행기관

The Royal Society of Chemistry

ISSN

2044-4753

ISSN

2044-4761

권

호

페이지

pp.2353-2358