초록
<P><B>Abstract</B></P> <P>Sugar alcohols (polyols) are sweeteners with many industrial applications. In this study, a fermentation process of polyol production based on waste substrates – raw industrial molasses and crude glycerol – was tested. The yeast strain <I>Yarrowia lipolytica</I> Wratislavia K1 was genetically modified by overexpression of the <I>Saccharomyces cerevisiae SUC2</I> gene and overexpression of the native <I>GUT1</I> gene. This process allowed for sucrose utilization and rapid glycerol assimilation by the engineered strain. In this study, the obtained strain AIB pAD-UTGut1 produced 100.65±3.75g/l of polyols, with productivity of 1.09±0.9g/lh and yield of 0.67±0.2g/g. This is the first study describing efficient polyol production by the modified <I>Y. lipolytica</I> strain from industrial raw molasses and crude glycerol. By process optimization, we established conditions for abundant polyol synthesis from low-value substrates.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The first study using wastes for efficient polyols synthesis by <I>Y. lipolytica.</I> </LI> <LI> Two-stage production results in short fermentation time. </LI> <LI> Raw molasses and crude glycerol promoted low-cost polyols production. </LI> </UL> </P>