The purpose of this study was to obtain the best slurry recirculation treatment for two-phase digester system which produced the highest biogas production in the DH-JcL substrate. The study was conducted in the laboratory using 750mL and 2 L of plastic digester and biofilter plastic as growth immobilize. The slurry recirculation treatment consisted of the recirculation of 50% slurry to methanogenesis digester, 50% slurry to hydrolysis digester, and 25% slurry to hydrolysis digester combined with 25% to methanogenesis digester. The highest daily biogas production was obtained from the recirculation of 50% slurry to hydrolysis digester. However, the best slurry recirculation treatment, which was more stable in longer period, was 50% slurry to methanogenesis digester.