BioChem - DOS

High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Lan, T.Q.; Gleisner, R.; Zhu, J.Y.; Dien, B.S.; Hector, R.E.

Lodgepole wood chips were pretreated by sulfite pretreatment to overcome recalcitrance of lignocelluloses (SPORL) at 25% solids loading and 180<SUP>o</SUP>C for 20min with sulfuric acid and sodium bisulfite charges of 2.2 and 8wt/wt% on an oven-dry wood basis, respectively. The pretreated wood chips were disk-milled with pretreatment spent liquor and water, and the solid fraction was separated from the liquor stream. The liquor was neutralized and concentrated through vacuum evaporation. Quasi-simultaneous enzymatic saccharification of the cellulosic solids and combined fermentation with the concentrated liquor was conducted at up to 20% total solids loading. Fed-batching of the solids facilitated liquefaction and saccharification, as well as managing instantaneous inhibitor concentrations. At a commercial cellulase (CTec2) loading of only 9 FPU or 0.06mL/g untreated wood, a maximum ethanol titer of 47.4g/L was achieved, resulting in a calculated yield of 285L/tonne of wood using Saccharomyces cerevisiae YRH400 at 35<SUP>o</SUP>C and pH 5.5.

2013

Elsevier Applied Science

0960-8524

127

pp.291-297

10.1016/j.biortech.2012.09.111

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65042893

Cellulosic ethanol; Biofuel and biorefinery; Fed-batch enzymatic saccharification and fermentation; Pretreatment; Woody biomass