BioChem - DOS

Improved Isolation of Microbiologically Produced (2R,3S)-Isocitric Acid by Adsorption on Activated Carbon and Recovery with Methanol

Organic process research & development

Aurich, Andreas; Hofmann, Jö rg; Oltrogge, Robert; Wecks, Mike; Glä ser, Roger; Blö mer, Laura; Mauersberger, Stephan; Mü ller, Roland A.; Sicker, Dieter; Giannis, Athanassios

<P>A new, efficient method for the isolation of (2<I>R</I>,3<I>S</I>)-isocitric acid (ICA) from its fermentation solution was developed. It is noteworthy that this method is based on selective adsorption directly from the fermentation solution on activated carbon, followed by the release of both ICA and citric acid by means of elution with methanol and their final separation by known methods. Thereby, several disadvantages were overcome: Electrodialysis is no longer necessary to remove cations such as Na<SUP>+</SUP> from the fermentation solution. Also, several hitherto accompanying dyestuffs were not observed with this method. Furthermore, removal of water by distillation is expendable. Eventually, the new crude product is of a quality that also avoids the use of a tedious slide vane rotary vacuum pump distillation of the trimethyl esters of both acids, which hitherto was the basis for the separation of ICA. In summary, the new method distinctly spares energy as well as time.</P><BR>[FIG OMISSION]</BR>

2017

American Chemical Society

1083-6160

1520-586x

21

6

pp.866-870

10.1021/acs.oprd.7b00090

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78035362

Activated carbon (AC); isocitric acid; yeast bioprocess; esterification; plant oil; Yarrowia lipolytica