초록
<P><B>Abstract</B></P> <P>In this study, the effect of ethylenediamine (EDA) on enzymatic hydrolysis with different cellulosic substrates and the approaches to reduce sugar loss in enzymatic hydrolysis were investigated. During enzymatic hydrolysis, xylose yield reduced 21.2%, 18.1% and 13.0% with 7.5mL/L EDA for AFEX pretreated corn stover (CS), washed EDA pretreated CS and CS cellulose. FTIR and GPC analysis demonstrated EDA reacted with sugar and produced high molecular weight (MW) compounds. EDA was prone to react with xylose other than glucose. H<SUB>2</SUB>O<SUB>2</SUB> and Na<SUB>2</SUB>SO<SUB>3</SUB> cannot prevent sugar loss in glucose/xylose-EDA mixture, although they inhibited the browning and high MW compounds formation. By decreasing temperature to 30°C, the loss of xylose yield reduced to only 3.8%, 3.6% and 4.2% with 7.5mL/L EDA in the enzymatic hydrolysis of AFEX pretreated CS, washed EDA pretreated CS and CS cellulose.</P> <P><B>Highlights</B></P> <P> <UL> <LI> The effect of ethylene diamine (EDA) on enzymatic hydrolysis was investigated. </LI> <LI> The Mailard reaction between EDA and carbohydrates led to sugar loss. </LI> <LI> The browning between EDA and sugar was weakened by Na<SUB>2</SUB>SO<SUB>3</SUB> and H<SUB>2</SUB>O<SUB>2</SUB>. </LI> <LI> Lower temperature during enzymatic hydrolysis reduced sugar loss. </LI> </UL> </P>