BioChem - DOS

Comparative production of cellulases by mutants of Penicillium janthinellum NCIM 1171 and its application in hydrolysis of Avicel and cellulose

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Singhvi, M.S.; Adsul, M.G.; Gokhale, D.V.

Mutants of Penicillium janthinellum NCIM 1171 were evaluated for cellulase production using both submerged fermentation (SmF) and solid state fermentation (SSF). Mutant EU2D-21 gave highest yields of cellulases in both SmF and SSF. Hydrolysis of Avicel and cellulose were compared using SmF and SSF derived enzyme preparations obtained from EU2D-21. Surprisingly, the use of SSF derived preparation gave less hydrolysis compared to SmF derived enzymes. This may be due to inactivation of &beta;-glucosidase at 50<SUP>o</SUP>C in SSF derived enzyme preparations. SmF derived enzyme preparations contained both thermostable and thermosensitive &beta;-glucosidases where as SSF derived enzyme preparations contained predominantly thermosensitive &beta;-glucosidase. This is the first report on less thermostability of SSF derived &beta;-glucosidase which is the main reason for getting less hydrolysis.

2011

Elsevier Applied Science

0960-8524

102

11

pp.6569-6572

10.1016/j.biortech.2011.01.014

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56032479

Solid state fermentation; β-Glucosidase thermostability; Mutant EU2D-21; Submerged fermentation