BioChem - DOS

Extreme-thermophilic biohydrogen production from lignocellulosic bioethanol distillery wastewater with community analysis of hydrogen-producing microflora

International journal of hydrogen energy

Qiu, C.; Wen, J.; Jia, X.

Extreme-thermophilic biohydrogen production from distillery wastewater was investigated in batch and continuous-mode operation. Hydrogen-producing mixed culture was enriched by repeated batch cultivations. Effect of temperature and pH on biohydrogen yield was investigated in batch experiments. The highest hydrogen yield of 196. mL/g-volatile solids<SUB>addded</SUB> (VS<SUB>added</SUB>) was obtained at 70 <SUP>o</SUP>C and pH 7.0 in batch culture. Continuous biohydrogen production was performed in CSTR reactor with yield of 172.0 mL/g-VS<SUB>added</SUB> at HRT (hydraulic retention time) of 4 days. The main metabolic products were acetate, lactate, and ethanol. Community structure of hydrogen-producing microflora was investigated by 16S rRNA gene sequence analysis. The microorganisms involved in both batch and continuous-mode operation were similar and hydrogen production was carried out by a group of extreme-thermophilic bacterial species related to Thermotoga, Coprothermobacter, Caldanaerobacter, Thermobrachium, and Caldicellulosiruptor.

2011

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

36

14

pp.8243-8251

10.1016/j.ijhydene.2011.04.089

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56514929

Extreme-thermophilic; Hydrogen production; Distillery wastewater; Mixed culture; 16S rRNA