BioChem - DOS

A simple fed-batch method for transcription and insect cell-free translation

Journal of bioscience and bioengineering

Sato, Y.; Aizawa, K.; Ezure, T.; Ando, E.; Uozumi, N.

We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.

2012

Society for Bioscience and Bioengineering, Japan ; Distributed outside Japan by Elsevier Science

1389-1723

1347-4421

114

6

pp.677-679

10.1016/j.jbiosc.2012.06.015

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART64798699

Insect cell-free system; Undigested plasmids; One batch reaction; Eukaryotic proteins; High-throughput