Development of Metabolically Engineered Corynebacterium glutamicum for Enhanced Production of Cadaverine and Its Use for the Synthesis of Bio-Polyamide 510
메타 데이터
바이오화학분류
바이오플라스틱
기타
바이오정밀화학
기타
화장품용 기능성소재
기타
의료용 화학소재
식품첨가제
논문
Development of Metabolically Engineered Corynebacterium glutamicum for Enhanced Production of Cadaverine and Its Use for the Synthesis of Bio-Polyamide 510
학술지
ACS sustainable chemistry et engineering
저자명
Kim, Hee Taek; Baritugo, Kei-Anne; Hyun, Sung Min; Khang, Tae Uk; Sohn, Yu Jung; Kang, Kyoung Hee; Jo, Seo Young; Song, Bong Keun; Park, Kyungmoon; Kim, Il-Kwon; Hwang, Yong Taek; Lee, Sang Yup; Park, Si Jae; Joo, Jeong Chan
초록
<P>Lysine decarboxylases (LDCs) from <I>Escherichia coli</I>, <I>Lactobacillus saerimneri</I>, <I>Streptomyces coelicolor</I>, <I>Selemonas ruminantium</I>, <I>Hafnia alvei</I>, and <I>Vibrio vulnificus</I> were examined for their ability to enhance the fermentative production of cadaverine in <I>Corynebacterium glutamcium</I>. Among these LDCs, the plasmid-based expression of the <I>H. alvei</I> LDC gene (<I>ldcC</I><SUB><I>Ha</I></SUB>) under strong promoters (P<SUB>H30</SUB>, P<SUB>H36</SUB>) produced high concentrations of cadaverine (11.4-11.5 g/L), which is similar to 12.5 g/L of cadaverine produced by the plasmid-based expression of the <I>E. coli</I> LDC gene (<I>ldcC</I><SUB><I>Ec</I></SUB>) by the P<SUB>H30</SUB> promoter in our previous report. The production of cadaverine in batch (30.8 g/L) and fed-batch (93.7 g/L) fermentation cultures using recombinant strain <I>Corynebacterium glutamicum</I> H30HaLDC expressing plasmid-borne <I>ldcC</I><SUB><I>Ha</I></SUB> under control of the P<SUB>H30</SUB> promoter was 18 and 14% higher than that obtained using <I>C. glutamicum</I> P-H30 expressing plasmid-borne <I>ldcC</I><SUB><I>Ec</I></SUB> under control of the P<SUB>H30</SUB> promoter, in which production was only 26 and 82.2 g/L in batch and fed-batch cultures, respectively. Finally, <I>C. glutamicum</I> GH30HaLDC was constructed by integration of <I>ldcC</I><SUB><I>Ha</I></SUB> into <I>C. glutamicum</I> PKC at the <I>lysE</I> site. <I>C. glutamicum</I> GH30HaLDC produced 125 g/L of cadaverine in fed-batch culture, which was 20% higher than the cadaverine production of 104 g/L by <I>C. glutamicum</I> G-H30 in our previous report. Cadaverine produced by fed-batch culture of <I>C. glutamicum</I> GH30HaLDC was purified and used for the synthesis of bio-based polyamide PA510 by copolymerization with sebacic acid. Synthesized PA510 showed thermal and material properties comparable to those of chemical-based PA510.</P><P>Recombinant <I>Corynebacterium glutamicum</I> is engineered to produce cadaverine for the synthesis of bio-polyamide 510.</P><BR>[FIG OMISSION]</BR>