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Non-targeted metabolomic reveals the effect of salt stress on global metabolite of halotolerant yeast Candida versatilis and principal component analysis

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바이오화학분류
    • 바이오플라스틱
      1. 고무
      2. 플라스틱
    • 바이오정밀화학
      1. 화학제품
    • 화장품용 기능성소재
      1. 기능성
    • 의료용 화학소재
      1. 건강보조식품
      2. 식품첨가제
논문

Non-targeted metabolomic reveals the effect of salt stress on global metabolite of halotolerant yeast Candida versatilis and principal component analysis

학술지

Journal of industrial microbiology & biotechnology

저자명

Qi, Wei; Fan, Zhen-Chuan; Wang, Chun-Ling; Hou, Li-Hua; Liu, Jin-Fu; Cao, Xiao-Hong

초록

<P><B>Abstract</B><P>As one of the major microbes in the soy sauce fermentation, Candida versatilis enriches the flavor and improves the quality of soy sauce. In this study, a combination of five different GC-MS and LC-MS-based metabolome analytical approaches was used to analyze the intracellular, extracellular and whole metabolites of C. versatilis. Our results found out that a total of 132, 244 and 267 different metabolites were detectable from the intracellular, extracellular and whole part, respectively. When exposed to 0. 9 and 18 % salt, respectively, 114, 123 and 129 different intracellular metabolites, 184, 200 and 178 extracellular metabolites and 177, 188 and 186 whole metabolites were detected, respectively. Our data showed that salt enhances the metabolic capacity of C. versatilis, especially its amino acid and enhances the synthesis and secretion of some metabolites of C. versatilis, especially the aldehydes and phenols, such as vanillin, guaiacol and 5-hydroxymethylfurfural. Our data also showed that special attention has to be paid to the generation of biogenic amines when C. versatilis was treated with salt.</P></P>

발행연도

2014

발행기관

Oxford University Press

ISSN

1367-5435

ISSN

1476-5535

41

10

페이지

pp.1553-1562

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논문; 2014-12-31

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