BioChem - DOS

Effect of Tween 80 on the growth, lipid accumulation and fatty acid composition of Thraustochytrium aureum ATCC 34304

Journal of bioscience and bioengineering

Taoka, Yousuke; Nagano, Naoki; Okita, Yuji; Izumida, Hitoshi; Sugimoto, Shinichi; Hayashi, Masahiro

<P><B>Abstract</B></P><P><I>Thraustochytrium aureum</I> ATCC 34304 was grown in the presence and absence of polyoxyethylene sorbitan monooleate (Tween 80). The aim of this work was to obtain basic knowledge about the effect of Tween 80 on growth, lipid accumulation and fatty acid composition in <I>T. aureum</I>. The addition of Tween 80 to a culture medium significantly enhanced the growth of <I>T. aureum</I>, and the biomass increased with an increase of Tween 80 content. Total lipid content and total fatty acid content were significantly higher in 1.0% Tween 80 in comparison with the control (absence of Tween 80). The fatty acid profile showed that the content of C18:1n-9 (oleic acid) significantly increased as a result of the addition of Tween 80. These results indicated that part of the Tween 80 added to the medium was utilized as a carbon source or that the oleate included in Tween 80 was directly incorporated into <I>T. aureum</I> cells as a fatty acid. Neither the DHA content nor the percentage of DHA did not change in spite of the addition of Tween 80. However, the DHA yield significantly increased because the biomass increased due to the addition of Tween 80.</P>

2011

Elsevier

1389-1723

1347-4421

111

4

pp.420-424

10.1016/j.jbiosc.2010.12.010

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55942403

Tween 80; Thraustochytrium aureum; Lipid accumulation; Docosahexaenoic acid (DHA); Thraustochytrids; Growth