Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source

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바이오화학분류

- 바이오플라스틱
  1. 고무
  2. 플라스틱
- 바이오정밀화학
  1. 용매
  2. 화학제품
- 화장품용 기능성소재
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- 의료용 화학소재
  1. 식품첨가제

논문

Succinate production by metabolically engineered Escherichia coli using sugarcane bagasse hydrolysate as the carbon source

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Liu, R.; Liang, L.; Cao, W.; Wu, M.; Chen, K.; Ma, J.; Jiang, M.; Wei, P.; Ouyang, P.

초록

Efficient biosynthesis of succinate from a renewable biomass resource by engineered Escherichia coli is reported in this paper. Fermentation of sugarcane bagasse hydrolysate by engineered E. coli BA204, a pflB, ldhA, and ppc deletion strain overexpressing the ATP-forming phosphoenolpyruvate carboxykinase from Bacillus subtilis 168, produced a final succinate concentration of 15.85gL-1 with a high yield of 0.89gL-1 total sugar under anaerobic conditions. During dual-phase fermentations, initial aerobic growth facilitated subsequent anaerobic succinate production, with a final succinate concentration of 18.88gL-1 and a yield of 0.96gg-1 total sugar after 24h of anaerobic fermentation. The high succinate yield from sugarcane bagasse hydrolysate demonstrated a great potential application of renewable biomass as a feedstock for the economical production of succinate using metabolically engineered E. coli.

발행연도

2013

발행기관

Elsevier Applied Science

ISSN

0960-8524

권

135

페이지

pp.574-577

DOI

10.1016/j.biortech.2012.08.120