<P><B>Abstract</B></P> <P>Diseased swines can be utilized as substrate for anaerobic fermentation after proper pretreatment. The cumulative bio-hydrogen and bio-methane production yields were taken as targets, the effects of different initial pH values and enzyme concentration on bio-hydrogen and bio-methane production characteristics by anaerobic fermentation from diseased swines were investigated. Results showed that the highest cumulative hydrogen yield reached up to 175.84 mL/L when pH value and enzyme concentration are of 8.0 and 2.5% respectively. While the maximum cumulative bio-methane-production yield of 104.59 mL/L was obtained when the enzyme concentration and pH value are of 1.0% and 8.0 respectively. The effects of enzyme on cumulative bio-hydrogen yield was greater than that of the initial pH while it is opposite for cumulative bio-methane yield. The potential of bio-hydrogen production from diseased swines is higher than that of bio-methane production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Potentials of bio-H<SUB>2</SUB> and bio-CH<SUB>4</SUB> production from diseased swine were compared. </LI> <LI> The highest cumulative bio-H<SUB>2</SUB> yield was 175.84 mL/L, 68% higher than bio-CH<SUB>4</SUB>. </LI> <LI> The effect of enzyme on cumulative bio-hydrogen yield was greater than that of pH. </LI> </UL> </P>