초록
<P><B>Abstract</B></P> <P>2-methyltetrahydrofuran, an ecofriendly substitute for traditional organic solvents, was employed to construct cosolvent system for synthesis of (S)-3-chloro-1-phenylpropanol by permeabilized recombinant <I>Escherichia coli</I> containing the YOL151W reductase and <I>Escherichia coli</I> containing <SMALL>D</SMALL>-glucose dehydrogenase to regenerate NADPH. By optimization, 30mM 3-chloro-1-phenyl-1-propanone could be efficiently converted to (S)-3-chloro-1-phenylpropanol with a yield of 98.6% and enantiomeric excess of more than 99.9% in 3% (v/v) of 2-methyltetrahydrofuran, 2% (v/v) of Triton X-100 and Tris-HCl buffer coslovent system. The application of 2-methyltetrahydrofuran has proved to be a promising cosolvent for asymmetric reduction of water-insoluble prochiral ketones.</P> <P><B>Highlights</B></P> <P> <UL> <LI> 2-Methyltetrahydrofuran was employed to construct cosolvent system. </LI> <LI> Synthesis of (S)-3-chloro-1-phenylpropanol was fulfilled in the cosolvent system. </LI> <LI> 2-Methyltetrahydrofuran can be a promising cosolvent for water-insoluble prochiral ketones. </LI> </UL> </P>