BioChem - DOS

Improved propionic acid production from glycerol with metabolically engineered Propionibacterium jensenii by integrating fed-batch culture with a pH-shift control strategy

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Zhuge, X.; Liu, L.; Shin, H.d.; Li, J.; Du, G.; Chen, J.

Propionic acid (PA) production with metabolically engineered Propionibacterium jensenii (pZGX04-gldA) was improved by integrating fed-batch culture with a two-stage pH control strategy in a 3-L fermenter. The following two-stage pH control strategy was used: the pH was controlled at 5.9 for 0-36h and shifted to 6.5 after 36h. The PA titer was increased to 21.43g/L. On the basis of pH control, the influence of fed-batch culture on PA production was further investigated and the maximum PA production (34.62g/L) was obtained when glycerol was fed at a constant rate of 3.33mL/h from 60 to 132h with an initial glycerol concentration of 25g/L. Crude glycerol was then used to produce PA using the optimized strategies, and maximal PA production reached 37.26g/L. The strategies may be useful for the production of PA by other propionibacteria species.

2014

Elsevier Applied Science

0960-8524

152

pp.519-525

10.1016/j.biortech.2013.11.063

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART68367137

Propionic acid; Propionibacterium jensenii; Metabolic engineering; Glycerol