BioChem - DOS

Optimization of cold-adapted alpha-galactosidase expression in Escherichia coli

Protein expression and purification

Golotin, V.A.; Balabanova, L.A.; Noskova, Yu.A.; Slepchenko, L.V.; Bakunina, I.Y.; Vorobieva, N.S.; Terenteva, N.A.; Rasskazov, V.A.

&alpha;-Galactosidase (&alpha;-PsGal) of the cold-adapted marine bacterium Pseudoalteromonas sp. KMM 701 was cloned into the pET-40b(+) vector to study its properties and to develop an effective method for modifying human B-erythrocytes into O-blood group. The use of heat-shock as a pre-induction treatment, IPTG concentration of 0.2 mM and post-induction cultivation at 18 <SUP>o</SUP>C for 20 h in the developed MX-medium allowed increasing the recombinant Escherichia coli Rosetta (DE3)/40Gal strain productivity up to 30 times and the total soluble &alpha;-PsGal yield up to 40 times.

2016

Academic Press

1046-5928

1096-0279

123

pp.14-18

10.1016/j.pep.2016.03.006

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75435242

Pseudoalteromonas sp.; Psychrophilic enzyme; Alpha-galactosidase; Prokaryotic gene expression