초록
<P><B>Abstract</B></P><P>A mutant strain of <I>Clostridium beijerinckii</I>, with high tolerance to ferulic acid, was generated using atmospheric pressure glow discharge and high‐throughput screening of <I>C. beijerinckii</I> NCIMB 8052. The mutant strain M11 produced 7.24 g/L of butanol when grown in P2 medium containing 30 g/L of glucose and 0.5 g/L of ferulic acid, which is comparable to the production from non‐ferulic acid cultures (8.11 g/L of butanol). When 0.8 g/L of ferulic acid was introduced into the P2 medium, <I>C. beijerinckii</I> M11 grew well and produced 4.91 g/L of butanol. Both cell growth and butanol production of <I>C. beijerinckii</I> M11 were seriously inhibited when 0.9 g/L of ferulic acid was added into the P2 medium. Furthermore, <I>C. beijerinckii</I> M11 could produce 6.13 g/L of butanol using non‐detoxified hemicellulosic hydrolysate from diluted sulfuric acid‐treated corn fiber (SAHHC) as the carbon source. These results demonstrate that <I>C. beijerinckii</I> M11 has a high ferulic acid tolerance and is able to use non‐detoxified SAHHC for butanol production.</P>