초록
<P><B>Abstract</B></P> <P>The aim of this work was to study the effects of deleting acetate transporter gene <I>ADY2</I> on growth and fermentation of <I>Saccharomyces cerevisiae</I> in the presence of inhibitors. Comparative transcriptome analysis revealed that three genes encoding plasma membrane carboxylic acid transporters, especially <I>ADY2</I>, were significantly downregulated under the zinc sulfate addition condition in the presence of acetic acid stress, and the deletion of <I>ADY2</I> improved growth of <I>S. cerevisiae</I> under acetic acid, ethanol and hydrogen peroxide stresses. Consistently, a concomitant increase in ethanol production by 14.7% in the presence of 3.6g/L acetic acid was observed in the <I>ADY2</I> deletion mutant of <I>S. cerevisiae</I> BY4741. Decreased intracellular acetic acid, ROS accumulation, and plasma membrane permeability were observed in the <I>ADY2</I> deletion mutant. These findings would be useful for developing robust yeast strains for efficient ethanol production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Zinc sulfate supplementation repressed transcription of acetate transporter gene <I>ADY2</I>. </LI> <LI> <I>ADY2</I> deletion improved yeast tolerance to acetic acid and hydrogen peroxide. </LI> <LI> <I>ADY2</I> deletion led to improved ethanol fermentation in the presence of acetic acid. </LI> <LI> Decreased intracellular acetic acid and ROS was revealed by <I>ADY2</I> deletion. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>