초록
A novel Clostridium tyrobutyricum strain RPT-4213 was found producing butyrate under strict anaerobic conditions. This strain produced 9.47gL<SUP>-1</SUP> butyric acid from MRS media (0.48g/g glucose). RPT-4213 was also used to ferment dilute acid pretreated hydrolysates including wheat straw (WSH), corn fiber (CFH), corn stover (CSH), rice hull (RHH), and switchgrass (SGH). Results indicated that 50% WSH with a Clostridia medium (Ct) produced the most butyric acid (8.06gL<SUP>-1</SUP>, 0.46g/g glucose), followed by 50% SGH with Ct (6.01gL<SUP>-1</SUP>, 0.44g/g glucose), however, 50% CSH Ct showed growth inhibition. RPT-4213 was then used in pH-controlled bioreactor fermentations using 60% WSH and SGH, with a dilute (0.5x) Ct medium, resulting 9.87gL<SUP>-1</SUP> butyric acid in WSH (yield 0.44g/g) and 7.05gL<SUP>-1</SUP> butyric acid in SGH (yield 0.42g/g). The titer and productivity could be improved through process engineering.