BioChem - DOS

16S rRNA in situ hybridization followed by flow cytometry for rapid identification of acetic acid bacteria involved in submerged industrial vinegar production

Food Technology and Biotechnology

Trček Janja; Department of Biology, Faculty of Natural Sciences and Mathematics, University of Maribor, SI-2000 Maribor, Slovenia; Lipoglavšek Luka; Avguštin Gorazd; Department of Animal Science, Biotechnical Faculty, University of Ljubljana, SI-1230 Domžale, Slovenia; 3Department of Animal Science, Biotechnical Faculty, University of Ljubljana, SI-1230 Domžale, Slovenia

Acetic acid bacteria are involved in many biotechnological processes such as vitamin C, gluconic acid, miglitol or acetic acid production, and others. For a technologist trying to control the industrial process, the ability to follow the microbiological development of the process is thus of importance. During the past few years hybridization in a combination with flow cytometry has oft en been used for this purpose. Since vinegar is a liquid, it is an ideal matrix for flow cytometry analysis. In this work we have constructed a specific probe for highly acetic acid-resistant species of the acetic acid bacteria and a protocol for in situ hybridization, which in combination with flow cytometry enables direct monitoring of bacteria producing vinegar with >10 % of acetic acid. The approach was successfully applied for monitoring microbiota during industrial vinegar production.

2016

Faculty of Food Technology and Biotechnology, University of Zagreb

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10.17113/ftb.54.01.16.4041

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=ATN0014162450