초록
<P><B>Abstract</B></P> <P>An aqueous two phase extraction using K<SUB>2</SUB>CO<SUB>3</SUB>:K<SUB>2</SUB>HPO<SUB>4</SUB>/Isoproponal was investigated for the recovery of 1,3-propanediol from the fermented broth. Initially, the concentration of K<SUB>2</SUB>CO<SUB>3</SUB> on phase formation, the partition co-efficient and recovery of 1,3-PDO was evaluated with a optimum salt concentration of 60%. Later the partition co-efficient was improved using dual inorganic salts, K<SUB>2</SUB>CO<SUB>3</SUB> and K<SUB>2</SUB>HPO<SUB>4</SUB> with an optimum concentration of 45% and 15% respectively. Using Central Composite Design, pH and temperature on partition and recovery of 1,3-PDO was evaluated. With the optimized physical conditions and inorganic salts concentration, ATPS extraction was carried out in synthetic solution as well as fermented broth resulting in maximum 1,3-PDO partition co-efficient value of 42.46 and 56.93 and recovery yield of 97.69 and 98.27% respectively. A fair partition was observed with organic acids and 1,3-PDO, with removal of lactic acid and acetic acid up to 93.29 and 90.42% respectively.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An ATPS extraction using dual inorganic electrolytes was observed to be an efficient process. </LI> <LI> Temperature has key role in increasing the partition co-efficient and recovery of 1,3-PDO. </LI> <LI> The process was efficient to remove cells and proteins from the fermented broth, hence simplify the downstream process. </LI> <LI> An average of 92% organic acid removal was observed. </LI> <LI> Selectivity of 1,3-PDO over organic acids was 11.65 fold which is an add on advantage for purification of 1,3-PDO. </LI> </UL> </P>