초록
<P>Solid state fermentation was carried out using various agro- industrial wastes with the best amylase producing strain isolated from soil. Different physicochemical conditions were varied for maximum enzyme production. The strain produced about 5400 units/g of amylase at 1:3 moisture content, 20% inoculum, after 72 h of incubation with Mustard Oil seed cake as the substrate. The optimum temperature and pH of the enzyme activity were found to be 50°C and 6 respectively. The enzyme was found to be thermostable at 70°C for about 2 h without any salt. It showed stability at pH range 5–7. The metal ions as Na<SUP>+</SUP>, Ca<SUP>++</SUP>, Mg<SUP>++</SUP> and Co<SUP>++</SUP> enhanced the enzyme activity.</P>