BioChem - DOS

Improvement of fermentative hydrogen production using genetically modified Enterobacter aerogenes

International journal of hydrogen energy

Zhao, Jinfang; Song, Wenlu; Cheng, Jun; Liu, Min; Zhang, Chuanxi; Cen, Kefa

<P><B>Abstract</B></P> <P>The [NiFe]-hydrogenase 3 of <I>Enterobacter aerogenes</I> contains a large subunit of 60 kDa (HycE) and a small subunit of 30 kDa (HycG). The gene of HycE and the gene of HycG were overexpressed in <I>E. aerogenes</I> separately by using the pGEX-4T-2-cat vector, to obtain two recombinant strains: <I>E. aerogenes</I>/HycE and <I>E. aerogenes</I>/HycG. The hydrogen yields were significantly enhanced by the recombinant strains. The hydrogenase activities of the recombinant strains increased by 124% (<I>E. aerogenes</I>/HycE) and 67% (<I>E. aerogenes</I>/HycG) compared with that of the wild strain. The hydrogen yields of the recombinant strains from initial glucose increased by 86.2% to 2.16 mol H<SUB>2</SUB>/mol (<I>E. aerogenes</I>/HycE) and by 69.8% to 1.97 mol H<SUB>2</SUB>/mol (<I>E. aerogenes</I>/HycG). The recombinant strains produced more acetate and butyrate than the wild strain, and this finding corresponded to the metabolism of NADH.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Genes of HycE and HycG were separately over-expressed in <I>Enterobacter aerogenes</I>. </LI> <LI> SDS-PAGE analysis confirms the successful expression of HycE and HycG. </LI> <LI> Hydrogenase activity of the recombinants is 124% and 67% higher than the wild strain. </LI> <LI> H<SUB>2</SUB> yields are 2.16 mol/mol of <I>E. aerogenes</I>/HycE and 1.97 mol/mol of <I>E. aerogenes</I>/HycG. </LI> <LI> The recombinants produce more acetate and butyrate as by-product, but less ethanol. </LI> </UL> </P>

2017

Elsevier

0360-3199

1879-3487

42

6

pp.3676-3681

10.1016/j.ijhydene.2016.08.161

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77442926

Enterobacter aerogenes; Hydrogenase; Overexpression; Hydrogen production; Metabolic analysis