초록
<P><B>Abstract</B></P> <P>This study investigated the cultivation of the marine alga <I>Nannochloropsis gaditana</I> in a medium based on desalination concentrate (DC) with an optimal concentration of 75% DC, under three trophic conditions and four photoperiod schedules. <I>N. gaditana</I> produced a peak biomass concentration (1.25gL<SUP>−1</SUP>) under mixotrophic culture condition and a photoperiod of 16L:08D. <I>N. gaditana</I> cells compensate to different light-dark regimes producing different amounts of protein (17.9–44.8%). The intracellular lipid content in <I>N. gaditana</I> cells increased both under autotrophic conditions with a 16L:08D cycle (16.7%), and under mixotrophic conditions with a 08L:16D cycle (15.7%). In heterotrophic culture, <I>N. gaditana</I> cells were rich in polyunsaturated fatty acids (46.0%). This study demonstrates an alternative approach to enhancing intracellular lipid content of the marine alga <I>N. gaditana</I> by modifying the photoperiod, trophic conditions and stress-salinity-conductivity with the use of a DC-based medium.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Optimal 75% DC concentration is appropriate medium for <I>N. gaditana</I> cultivation. </LI> <LI> DC provided a higher lipid productivity than the control medium. </LI> <LI> The optimal photoperiod cycles for enhancing algal biomass were determined. </LI> <LI> The use of the optimum DC medium was evaluated in nine treatments. </LI> <LI> Higher SFA production was observed under autotrophic than heterotrophic condition. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>