BioChem - DOS

Complete Enzymatic Oxidation of Methanol to Carbon Dioxide: Towards More Eco-Efficient Regeneration Systems for Reduced Nicotinamide Cofactors

Advanced synthesis & catalysis

Kara, Selin; Schrittwieser, Joerg H.; Gargiulo, Serena; Ni, Yan; Yanase, Hideshi; Opperman, Diederik J.; van  Berkel, Willem J. H.; Hollmann, Frank

<P><B>Abstract</B></P><P>A novel system for <I>in situ</I> regeneration of reduced nicotinamide cofactors (NADH) is proposed: through a cascade of alcohol dehydrogenase (ADH), formaldehyde dismutase (FDM) and formate dehydrogenase (FDH) complete oxidation of methanol to carbon dioxide (CO<SUB>2</SUB>) is coupled to the regeneration of NADH. As a consequence, from one equivalent of methanol three equivalents of NADH can be obtained. The feasibility of this cascade is demonstrated at the examples of an NADH&#8208;dependent reduction of conjugated C&#63742;C&#8208;double bonds (catalysed by an enoate reductase) and the NADH&#8208;dependent hydroxylation of phenols (catalysed by a monooxygenase). The major limitation of the current regeneration system is the comparably poor catalytic efficiency of the methanol oxidation step (low <I>k</I><SUB>cat</SUB> and high <I>K</I><SUB>M</SUB> value of the ADH used) necessitating higher than theoretical methanol concentrations.</P>

2015

1615-4150

1615-4169

357

8

pp.1687-1691

10.1002/adsc.201500173

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72118381

cofactor regeneration; complete methanol oxidation; enzymatic cascades; oxidoreductases