Sulfide impact on biohydrogen production using dark fermentation of glucose at 37<SUP>o</SUP>C was investigated. Dissolved sulfide (S<SUP>2-</SUP>) at a low concentration (25mg/L) increased biohydrogen production by 54% relative to the control (without iron addition). Whereas on initial dissolved S<SUP>2-</SUP> concentration of 500mg/L significantly inhibited the biohydrogen production with total cumulative biohydrogen decreasing by 90% compared to the control (without iron addition). At sulfide concentrations of 500mg S<SUP>2-</SUP>/L, addition of Fe<SUP>2+</SUP> at 3-4 times the theoretical requirement to precipitate 100% of the dissolved S<SUP>2-</SUP> entirely eliminated the inhibitory effect of sulfide.