BioChem - DOS

Towards an extractive bioconversion of 3-hydroxypropionic acid: study of inhibition phenomena

Journal of chemical technology and biotechnology

Burgé , Gregoire; Moussa, Marwen; Saulou‐ Bé rion, Claire; Chemarin, Florian; Kniest, Mariane; Allais, Florent; Spinnler, Henry‐ Eric; Athè s, Violaine

<P><B>Abstract</B></P><P><B>BACKGROUND</B></P><P>3&#8208;hydroxypropionic acid (3&#8208;HP) microbial synthesis through glycerol bioconversion by <I>Lactobacillus reuteri</I> is at the moment characterized by too low performances to consider production at industrial scale. To avoid its toxic accumulation in the medium and to recover this molecule of interest, 3&#8208;HP <I>in situ</I> reactive extraction from bioconversion broth was investigated using a hollow fiber membrane contactor (HFMC) in order to intensify its production. The so&#8208;called integrated system was compared with the conventional bioconversion system. The impact of the extractive bioconversion on the overall production performance and on cell physiological state was studied.</P><P><B>RESULTS</B></P><P>Results underlined drastic inhibitory effects on the producing bacteria, especially under extractive bioconversion conditions despite the use of a HFMC supposed to avoid direct contact between organic phase and bacteria. Indeed, the extractant phase components (trioctylamine in <I>n</I>&#8208;decanol) were found to be toxic for the cells (due to solubility and by direct contact). These phenomena were increased by the presence of 3&#8208;hydroxypropionaldehyde (3&#8208;HPA) and 3&#8208;HP produced during glycerol bioconversion. These cumulative effects induced complete loss of the cell membrane integrity and esterase activity after 1.5 h of extractive bioconversion. When the bioconversion was conducted alone, the bacterial inhibition was lower, as around 50% of <I>L. reuteri</I> cells remained active with unaltered membrane after 3 h. Hypotheses concerning the mechanisms of action of the observed inhibitions were proposed and discussed.</P><P><B>CONCLUSIONS</B></P><P>This work highlighted the importance of performing such an integrative approach to address specific questions prior to the optimization of the whole process. &copy; 2017 Society of Chemical Industry</P>

2017

John WileySons, Ltd

cc-by-sa

0268-2575

1097-4660

92

9

pp.2425-2432

10.1002/jctb.5253

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78277774

https://hal.archives-ouvertes.fr/hal-01566310/file/Burg-_et_al-2017-Journal_of_Chemical_Technology_and_Biotechnology_%7BB478A98A-8B45-4CED-9B36-B27224EB2324%7D.pdf

3&#x2010; hydroxypropionic acid (3&#x2010; HP); Lactobacillus reuteri; in situ product recovery (ISPR); hollow fiber membrane contactor (HFMC); inhibition; glycerol bioconversion;