초록
<P>L-Proline hydroxylase is a member of the non-heme Fe<SUP>2+</SUP>/α-ketoglutarate (AKG)-dependent hydroxylase family that catalyzes the reaction from L-proline to hydroxy-L-proline, which is widely used in drug synthesis, biochemistry, food supplementation and cosmetic industries. Here, the first crystal structure of L-proline <I>trans</I>-hydroxylase and its complexes with substrate and product are reported, which reveal the structural basis of <I>trans-cis</I> proline hydroxylation selectivity. Structure comparison with other AKG-dependent hydroxylases identifies conserved amino acid residues, which may serve as signatures of in-line or off-line AKG binding modes in the AKG-dependent enzyme family.</P>