초록
Carotenoids profile of the unicellular Eustigmatos cf. polyphem (Eustigmatophyceae) and β-carotene production of the microalga in bubble column and large flat panel bioreactors were studied. The microalga which contained β-carotene, violaxanthin and vaucheriaxanthin as the major carotenoids accumulated large amount of β-carotene. The β-carotene production of this microalga in the bubble column bioreactor was considerable, with the maximum intracellular β-carotene content reaching 60.76mgg<SUP>-1</SUP>, biomass reaching 9.2gL<SUP>-1</SUP>, and β-carotene yield up to 470.2mgL<SUP>-1</SUP>. The β-carotene productions in two large flat panel bioreactors were relatively lower, whereas over 100mg β-carotene L<SUP>-1</SUP> was achieved. Besides, high light intensity helped to accumulate intracellular β-carotene and biomass. Deficient nitrate supply inhibited biomass growth, though it helped to accumulate β-carotene. Our results first proved that E. cf. polyphem was a potential source and producer of β-carotene, making it an interesting subject for further β-carotene study or commercial exploration.