BioChem - DOS

Raffinose production using α-galactosidase from Paraphaeosphaeria sp.

Mycoscience

Nakata, H.; Sakurai, H.; Nagura, T.; Tanaka, M.; Sone, T.; Asano, K.

An &alpha;-galactosidase for raffinose synthesis by a reverse hydrolysis reaction was searched from intracellular of endophytic fungi. About 500 strains were screened, one strain was selected and identified as Paraphaeosphaeria sp. Partially purified &alpha;-galactosidase (1 U/ml in the reaction mixture), 100 g/L galactose, and 500 g/L sucrose at 60 <SUP>o</SUP>C for 48 h resulted in synthesis of 13.3 g/L raffinose and 4.6 g/L planteose at a ratio of about 3:1. The data suggest that &alpha;-galactosidase was able to synthesize raffinose at a ratio higher than that of &alpha;-galactosidases derived from other fungi.

2013

Mycological Society of Japan

1340-3540

1618-2545

54

4

pp.247-251

10.1016/j.myc.2012.09.016

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65990609

Endophytic fungi; Intracellular glycosidase; Planteose; Reverse hydrolysis reaction