초록
Biotransformation of long-chain fatty acids into medium-chain ,-dicarboxylic acids or -aminocarboxylic acids could be achieved with biocatalysts. This study presents the production of ,-dicarboxylic acids (e.g., C9, C11, C12, C13) and -aminocarboxylic acids (e.g., C11, C12, C13) directly from fatty acids (e.g., oleic acid, ricinoleic acid, lesquerolic acid) using recombinant Escherichia coli-based biocatalysts. -Hydroxycarboxylic acids, which were produced from oxidative cleavage of fatty acids via enzymatic reactions involving a fatty acid double bond hydratase, an alcohol dehydrogenase, a Baeyer-Villiger monooxygenase and an esterase, were then oxidized to ,-dicarboxylic acids by alcohol dehydrogenase (ADH, AlkJ) from Pseudomonas putida GPo1 or converted into -aminocarboxylic acids by a serial combination of ADH from P. putida GPo1 and an -transaminase of Silicibacter pomeroyi. The double bonds present in the fatty acids such as ricinoleic acid and lesquerolic acid were reduced by E. coli-native enzymes during the biotransformations. This study demonstrates that the industrially relevant building blocks (C9 to C13 saturated ,-dicarboxylic acids and -aminocarboxylic acids) can be produced from renewable fatty acids using biocatalysis.