초록
Xylanolytic Bacillus pumilus SS1 grew well at highly alkaline pH (9-11) and at moderately high temperature (25-55<SUP>o</SUP>C) and successfully utilized wheat bran as the sole carbon source and produced considerable titer of xylanase. Maximum enzyme production occurred at medium pH 8 and at 45<SUP>o</SUP>C. The enzyme purified (2.97-fold) with ammonium sulfate fractionation and carboxymethyl sephadex chromatography, showed molecular weight of 25kDa, low K<SUB>m</SUB> (2.7mg/ml) and reasonably good V<SUB>max</SUB> (36μmol/mg/min). Enzyme showed maximum activity at pH 6-8, and at 40-50<SUP>o</SUP>C. Enzyme was inhibited by HgCl<SUB>2</SUB>, CoCl<SUB>2</SUB>, MnSO<SUB>4</SUB>, MgCl<SUB>2</SUB>, phenylmethylsulphonylfluoride and CaCl<SUB>2</SUB>, while FeCl<SUB>2</SUB> caused slight stimulation.