초록
In this study, sweet sorghum bagasse was used as an immobilized carrier for acetone-butanol-ethanol (ABE) fermentation production. Scanning electron microscopy revealed the relationship between<I>Clostridium</I>cells and the sorghum bagasse in terms of adsorption and embedding. The ABE productivity and yield of ABE solvents in batch fermentation were 0.37 g L<SUP>−1</SUP>h<SUP>−1</SUP>and 0.41 g g<SUP>−1</SUP>, with 68% and 24% improvement than free cells, respectively. Repeated batch fermentation was carried out under optimized conditions, plus a total of 970 h of continuous fermentation at different dilution rates. A maximum ABE concentration of 16.5 g L<SUP>−1</SUP>was obtained at a dilution rate of 0.08 per h, with optimized yield and productivity. This novel immobilization method using sweet sorghum bagasse offers an attractive prospect for the industrial production of bio-based butanol..