초록
<P>We demonstrated direct utilization of xylooligosaccharides using β-xylosidase-displaying <I>Escherichia coli</I>. After screening active β-xylosidases, BSU17580 from <I>Bacillus subtilis</I> or Tfu1616 from <I>Thermobifida fusca</I> YX, were successfully displayed on the <I>E. coli</I> cell surface using Blc or HdeD as anchor proteins, and these transformants directly assimilated xylooligosaccharides as a carbon source. The final OD 600 in minimal medium containing 2% xylooligosaccharides was 1.09 (after 12 h of cultivation) and 1.30 (after 40 h of cultivation). We then constructed an <I>E. coli</I> strain displaying both β-glucosidase and β-xylosidase. β-glucosidase- and β-xylosidase-displaying <I>E. coli</I> was successfully grown on a 1% cellobiose and 1% xylooligosaccharides mixture, and the OD 600 was 1.76 after 10 h of cultivation, which was higher and reached faster than that grown on a glucose/xylose mixture (1.20 after 30 h of cultivation).</P><P><B>Graphic Abstract</B><BR><IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/asbcd6/2014/asbcd6.2014.3.issue-7/sb400070q/production/images/medium/sb-2013-00070q_0006.gif'></P>