초록
<P><B>Highlights</B></P><P>► Paper tube residual was investigated as a potential raw material for ethanol production. ► The chemical compositions of paper tube residuals were determined. ► The effects of two pretreatment methods on the enzymatic hydrolysis and SSF were studied. ► Pretreatment with concentrate phosphoric acid was found as an appropriate method for maximum ethanol production. ► Under optimal condition, 308ml ethanol per kg paper tube could be produced.</P> <P><B>Abstract</B></P><P>Paper tube residual was utilized as a raw material for ethanol production. The effects of two pretreatment methods namely dilute acid steam explosion (DASE) and concentrate phosphoric acid (CPA) on enzymatic hydrolysis and SSF were studied. Cellulose, lignin, glue (PVA), and xylan were the main components of paper tube accounting for 52%, 20%, 9% and 7% of dry matter, respectively. Presence of PVA delayed the growth of yeast cells but showed no effect on ultimate yield of ethanol. Higher cellulase concentration as well as pretreatments increased hydrolysis rate and ultimate yield of ethanol. Enzymatic hydrolysis of native paper tube for 72h resulted in 49% of theoretical glucose conversion while pretreatments by DASE and CPA increased this value to 67% and 93%, respectively. The best result of SSF process was from the CPA-pretreated paper tubes with an ethanol yield of 0.42g/g after 48h. Under optimal condition, 308ml ethanol per kg paper tube could be produced.</P>