초록
<P>A novel analytical system was developed to rapidly and accurately quantify total volatile organic compound (VOC) production from microbial reactor systems using a platinum catalyst and a sensitive CO<SUB>2</SUB> detector. This system allows nearly instantaneous determination of total VOC production by utilizing a platinum catalyst to completely and quantitatively oxidize headspace VOCs to CO<SUB>2</SUB> in coordination with a CO<SUB>2</SUB> detector. Measurement of respiratory CO<SUB>2</SUB> by bypassing the catalyst allowed the total VOC content to be determined from the difference in the two signals. To the best of our knowledge, this is the first instance of a platinum catalyst and CO<SUB>2</SUB> detector being used to quantify the total VOCs produced by a complex bioreactor system. Continuous recording of these CO<SUB>2</SUB> data provided a record of respiration and total VOC production throughout the experiments. Proton transfer reaction-mass spectrometry (PTR-MS) was used to identify and quantify major VOCs. The sum of the individual compounds measured by PTR-MS can be compared to the total VOCs quantified by the platinum catalyst to identify potential differences in detection, identification and calibration. PTR-MS measurements accounted on average for 94 % of the total VOC carbon detected by the platinum catalyst and CO<SUB>2</SUB> detector. In a model system, a VOC producing endophytic fungus <I>Nodulisporium</I> isolate TI-13 was grown in a solid state reactor utilizing the agricultural byproduct beet pulp as a substrate. Temporal changes in production of major volatile compounds (ethanol, methanol, acetaldehyde, terpenes, and terpenoids) were quantified by PTR-MS and compared to the total VOC measurements taken with the platinum catalyst and CO<SUB>2</SUB> detector. This analytical system provided fast, consistent data for evaluating VOC production in the nonhomogeneous solid state reactor system.</P><P><B>Electronic supplementary material</B></P><P>The online version of this article (doi:10.1186/s13568-016-0264-2) contains supplementary material, which is available to authorized users.</P>