BioChem - DOS

Extractive fermentation for improved production and recovery of lipase derived from Burkholderia cepacia using a thermoseparating polymer in aqueous two-phase systems

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Show, P.L.; Tan, C.P.; Shamsul Anuar, M.; Ariff, A.; Yusof, Y.A.; Chen, S.K.; Ling, T.C.

An extractive fermentation technique was developed using a thermoseparating reagent to form a two-phase system for simultaneous cell cultivation and downstream processing of extracellular Burkholderia cepacia lipase. A 10% (w/w) solution of ethylene oxide-propylene oxide (EOPO) with a molecular mass of 3900g/mol and pH 8.5, a 200rpm speed, and 30<SUP>o</SUP>C were selected as the optimal conditions for lipase production (55U/ml). Repetitive batch fermentation was performed by continuous replacement of the top phase every 24h, which resulted in an average cell growth mass of 4.7g/L for 10 extractive batches over 240h. In scaling-up the process, a bench-scale bioreactor was tested under the conditions that had been optimized in flasks. The production rate and recovery yield were higher in the bioreactor compared to fermentation performed in flasks.

2012

Elsevier Applied Science

0960-8524

116

pp.226-233

10.1016/j.biortech.2011.09.131

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63204161

Lipase; Thermoseparation; Aqueous two-phase systems (ATPS); Ethylene oxide-propylene oxide (EOPO); Recovery