BioChem - DOS

Efficient saccharification for non-treated cassava pulp by supplementation of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Vaithanomsat, P.; Kosugi, A.; Apiwatanapiwat, W.; Thanapase, W.; Waeonukul, R.; Tachaapaikoon, C.; Pason, P.; Mori, Y.

Cassava pulp containing 60% starch and 20% cellulose is a promising renewable source for bioethanol. The starch granule was observed to tightly bind cellulose fiber. To achieve an efficient degradation for cassava pulp, saccharification tests without pre-gelatinization treatment were carried out using combination of commercial α-amylase with cellulosome from Clostridium thermocellum S14 and β-glucosidase (rCglT) from Thermoanaerobacter brockii. The saccharification rate for cassava pulp was shown 59% of dry matter. To obtain maximum saccharification rate, glucoamylase (GA) from C. thermocellum S14 was supplemented to the combination. The result showed gradual increase in the saccharification rate to 74% (dry matter). Supplementation of GA to the combination of commercial α-amylase, cellulosome and rCglT is powerful method for efficient saccharification of cassava pulp without pretreatment.

2013

Elsevier Applied Science

0960-8524

132

pp.383-386

10.1016/j.biortech.2012.11.023

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART65633155

Saccharification; Non-treated cassava pulp; Cellulosome; Thermophilic β-glucosidase; Amylolytic enzymes;