초록
<P><B>Abstract</B></P> <P>In this work, the consolidated bioprocessing (CBP) yeast <I>Saccharomyces cerevisiae</I> MNII/cocδBEC3 was transformed by an artificial zinc finger protein (AZFP) library to improve its thermal tolerance, and the strain MNII-AZFP with superior growth at 42°C was selected. Improved degradation of acid swollen cellulose by 45.9% led to an increase in ethanol production, when compared to the control strain. Moreover, the fermentation of Jerusalem artichoke stalk (JAS) by MNII-AZFP was shortened by 12h at 42°C with a concomitant improvement in ethanol production. Comparative transcriptomics analysis suggested that the AZFP in the mutant exerted beneficial effect by modulating the expression of multiple functional genes. These results provide a feasible strategy for efficient ethanol production from JAS and other cellulosic biomass through CBP based-fermentation at elevated temperatures.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Artificial zinc finger protein library was transformed into a CBP yeast strain. </LI> <LI> A thermotolerant CBP yeast strain was selected which can endure up to 42°C. </LI> <LI> Jerusalem artichoke stalks (JAS) was utilized for ethanol production by CBP. </LI> <LI> Ethanol production at high temperature was achieved using the mutant from JAS. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>