BioChem - DOS

Engineering of formate dehydrogenase for improving conversion potential of carbon dioxide to formate

World journal of microbiology & biotechnology

Shi, Hong-Ling; Yuan, Shu-Wei; Xi, Xiao-Qi; Xie, Yu-Li; Yue, Chao; Zhang, Ying-Jun; Yao, Lun-Guang; Xue, Chuang; Tang, Cun-Duo

Formate dehydrogenase (FDH) is a D-2-hydroxy acid dehydrogenase, which can reversibly reduce CO2 to formate and thus act as non-photosynthetic CO2 reductase. In order to increase catalytic efficiency of formate dehydrogenase for CO2 reduction, two mutants V328I/F285W and V354G/F285W were obtained of which reduction activity was about two times more than the parent CbFDHM2, and the formate production from CO2 catalyzed by mutants were 2.9 and 2.7-fold higher than that of the parent CbFDHM2. The mutants had greater potential in CO2 reduction. The optimal temperature for V328I/F285W and V354G/F285W was 55 °C, and they showed increasement of relative activity under 45 °C to 55 °C compared with parent. The optimal pH for the mutants was 9.0, and they showed excellent stability in pH 4.0-11.5. The kcat/Km values of mutants were 1.75 times higher than that of the parent. Then the molecular basis for its improvement of biochemical characteristics were preliminarily elucidated by computer-aided methods. All of these results further established a solid foundation for molecular modification of formate dehydrogenase and CO2 reduction.

2023

Springer-Verlag

0959-3993

1573-0972

39

12

pp.352

10.1007/s11274-023-03739-5

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