Enhanced hydrogen production through co-cultivation of Chlamydomonas reinhardtii CC-503 and a facultative autotrophic sulfide-oxidizing bacterium under sulfurated conditions
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Enhanced hydrogen production through co-cultivation of Chlamydomonas reinhardtii CC-503 and a facultative autotrophic sulfide-oxidizing bacterium under sulfurated conditions
<P><B>Abstract</B></P> <P>Photoproduction of H<SUB>2</SUB> using microalgae has been considered as a promising approach for developing sustainable hydrogen energy. The algae <I>C. reinhardtii</I> CC-503 was co-cultured with a facultative autotroph sulfur-oxidizing bacterium <I>Thuomonas intermedia</I> BCRC 17547 to improve H<SUB>2</SUB> production. The maximum H<SUB>2</SUB> production of co-culture at sulfur deficiency conditions was 122 μmol/mg Chl with algae/bacteria ratio as 60:1, which was 2.8-fold higher than that of the pure algal culture. Na<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB> treatment can result in a maximum H<SUB>2</SUB> photoproduction rate of 255 μmol/mg Chl, which was 5.9 and 2.1 times higher than those of pure algae culture and co-culture without Na<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB>. Co-cultivation under sulphate condition can also significantly increase the biomass, respiratory rate, starch content and hydrogenase activity of <I>C. reinhardtii</I>. By supplement of Na<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB>, persistent (52 days) H<SUB>2</SUB> production of bacteria/algae co-culture can be achieved. Our results demonstrated that co-culture of <I>C. reinhardtii</I> CC-503 and bacteria BCRC17547 is a cost-effective strategy for improving photobiological H<SUB>2</SUB> production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Co-culture of <I>C. reinhardtii</I> and BCRC17547 can increase H<SUB>2</SUB> production and duration time. </LI> <LI> The H<SUB>2</SUB> production and biomass of co-culture can be enhanced by Na<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB> treatment. </LI> <LI> Persistent H<SUB>2</SUB> production can be achieved by fed-batch supplement of Na<SUB>2</SUB>S<SUB>2</SUB>O<SUB>3</SUB>. </LI> <LI> The increased respiratory rate, starch content and H<SUB>2</SUB>ase activity benefit for H<SUB>2</SUB> production. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>