BioChem - DOS

Directional regulation of the metabolic heterogeneity in anaerobic mixed culture to enhance fermentative hydrogen production by adaptive laboratory evolution

International journal of hydrogen energy

Huang, Z.; Yu, J.; Xiao, X.; Miao, H.; Ren, H.; Zhao, M.; Ruan, W.

In this study, an adaptive laboratory evolution strategy was originally developed to enhance fermentative hydrogen production by directionally regulating the metabolic heterogeneity in anaerobic mixed culture. The results indicated that the co-introduction of 4-methylpyrazole and oxamate could redistribute the metabolic flux to butyrate-type hydrogen fermentation. Subsequently, a synergistic evolutionary pressure, combining exogenous butyrate stress with 4-methylpyrazole and oxamate, was employed to evolve hydrogen-producing mixed culture with continuous fermentation system. The metabolic engineering strategy could directionally regulate the metabolic heterogeneity through efficiently shaping powerful butyrate-type hydrogen-producing community, by which evolved culture acquired a significantly improved hydrogen yield and productivity. Furthermore, compared with original culture, evolved culture possessed much higher activities of pyruvate-ferredoxin oxidoreductase and hydrogenase but a much lower ferredoxin-NAD<SUP>+</SUP> oxidoreductase activity, and these enzymatic evolutionary mechanisms were crucially important for the enhanced hydrogen fermentation.

2016

Pergamon Press ; Elsevier Science Ltd

0360-3199

1879-3487

41

24

pp.10145-10151

10.1016/j.ijhydene.2016.05.012

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75760217

Metabolic heterogeneity; Anaerobic mixed culture; Fermentative hydrogen production; Adaptive laboratory evolution