BioChem - DOS

Asymmetric synthesis of optically active methyl-2-benzamido-methyl-3-hydroxy-butyrate by robust short-chain alcohol dehydrogenases from Burkholderia gladioli

Chemical communications : Chem comm

Chen, Xiang; Liu, Zhi-Qiang; Huang, Jian-Feng; Lin, Chao-Ping; Zheng, Yu-Guo

<P>Three short-chain alcohol dehydrogenases from <I>Burkholderia gladioli</I> were discovered for their great potential in the dynamic kinetic asymmetric transformation of methyl 2-benzamido-methyl-3-oxobutanoate, and their screening against varied organic solvents and substrates. This is the first report of recombinant enzymes capable of achieving this reaction with the highest enantio- and diastereo-selectivity.</P><BR><BR><P>Graphic Abstract</P><P>Specific short-chain alcohol dehydrogenases were discovered and used in the dynamic kinetic asymmetric transformation of methyl 2-benzamido-methyl-3-oxobutanoate with excellent stereo-selectivity.<BR><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c5cc04652a'><BR></P>

2015

The Royal Society of Chemistry

1359-7345

1364-548x

51

61

pp.12328-12331

10.1039/c5cc04652a

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72430465