초록
Culture conditions for enhanced cellulase production from a newly isolated brown rot fungus, Fomitopsis sp. RCK2010 were optimized under solid state fermentation. An initial pH of 5.5 and moisture ratio of 1:3.5 (solid:liquid) were found to be optimal for maximum enzyme production. Of the different carbon sources tested wheat bran gave the maximum production of CMCase (71.526IU/g), FPase (3.268IU/g), and β-glucosidase (50.696IU/g). Among the nitrogen sources, urea caused maximum production of CMCase (81.832IU/g), where as casein and soyabean meal gave the highest FPase (4.682IU/g) and β-glucosidase (69.083IU/g) production, respectively. Among amino acids tested glutamic acid gave the highest production for CMCase (84.127IU/g); however 4-hydroxy-l-proline stimulated maximum FPase production (6.762IU/g). Saccharification of pretreated rice straw and wheat straw by crude enzyme extract from Fomitopsis sp. RCK2010 resulted in release of 157.160 and 214.044mg/g of reducing sugar, respectively.