BioChem - DOS

Oxidative Decarboxylation of Short-Chain Fatty Acids to 1-Alkenes

Angewandte Chemie. international edition

Dennig, Alexander; Kuhn, Miriam; Tassoti, Sebastian; Thiessenhusen, Anja; Gilch, Stefan; Bü lter, Thomas; Haas, Thomas; Hall, Mé lanie; Faber, Kurt

<P><B>Abstract</B></P><P>The enzymatic oxidative decarboxylation of linear short&#8208;chain fatty acids (C4:0&ndash;C9:0) employing the P450&#8197;monooxygenase OleT, O<SUB>2</SUB> as the oxidant, and NAD(P)H as the electron donor gave the corresponding terminal C<SUB>3</SUB> to C<SUB>8</SUB>&#8197;alkenes with product titers of up to 0.93&#8197;g&thinsp;L<SUP>&minus;1</SUP> and TTNs of >2000. Key to this process was the construction of an efficient electron&#8208;transfer chain employing putidaredoxin CamAB in combination with NAD(P)H recycling at the expense of glucose, formate, or phosphite. This system allows for the biocatalytic production of industrially important 1&#8208;alkenes, such as propene and 1&#8208;octene, from renewable resources for the first time.</P>

2015

1433-7851

1521-3773

54

30

pp.8819-8822

10.1002/anie.201502925

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72377501

1&#8208; alkenes; biocatalysis; fatty acids; OleT; oxidative decarboxylation;